@conference {164,
	title = {Isolasi dan Pencirian Xilanase dari Bakteri asal Tanah Hutan Taman Nasional Bukit Duabelas, Jambi, Indonesia/Isolation and Characterization Xylanase from Bacterial Origin Bukit Duabelas National Park Forest Soil, Jambi, Indonesia},
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {Xilanase merupakan enzim pendegradasi xilan yang berguna dalam penyempurnaan biokon versi biomassa lignoselulosa menjadi produk bermanfaat. Aplikasi xilanase yang luas belum dioptimalkan karena kurang tersedianya mikroba unggul. Penelitian inibertujuanuntukmengisolasibakteriasaltanahhutan Taman Nasional Bukit Duabelas, Jambi danmencirikanxilanasenya yang meliputi parameter pH optimum, suhu optimum, serta stabilitasnya. Sebanyak 28 isolat potensial telah berhasil diisolasi dari tanah hutan Taman Nasional Bukit Duabelas, Jambi dan tiga di antaranya berhasil dicirikan xilanasenya (isolat 10, 18, dan 27). Isolat 10 memproduksi xilanase ekstra seluler tertinggi pada jam ke-12 dan jam ke-36 inkubasi kultur dengan aktivitas optimum pada pH 6 di suhu 40oC dan 90oC.Waktu produk sixilanase tertinggi isolat 27 tercapai pada jam ke-12 dan jam ke-30 inkubasi kultur. Kondisi optimum xilanase isolat 27 terjadi pada pH 5 disuhu 90oC dan 50oC, sementara xilanase isolat 18 mencapai waktu optimum produksi pada jam ke-18 dan jam ke-36 inkubasi kultur. Kondisi optimum xilanase isolat 18 tercapai pada pH 5 dan suhu 90oC.Enzim isolat 18 memiliki waktu paruh 1 jam 7 menit pada pH dan suhu optimumnya.

Xylanase is a xylan-degrading enzymes which are useful to improving the bioconversion of lignocellulosic biomass into useful products. Broad application of xylanase have not optimized due to the lack of superior microbes. The aim of this study is to isolate bacterial origin Bukit Duabelas National Park, Jambi and characterize its xylanase, including its pH optimum, temperature optimum, and stability. A total of 28 potential isolates have been isolated from Bukit DuabelasNational Park soil, Jambi and three were successfully characterized its xilanase (isolates 10, 18, and 27). Isolate 10 reached the highest producing extracellularxylanase at 12th and 36th hours of culture incubation with optimum activity at pH 6, temperature 40 {\textdegree}C and 90oC. Isolate 27 reached its highest producing extracellular xylanase at 12th and 30th hours of culture incubation. The optimum condition of xylanase isolate 27 occurred at pH 5, temperature 90oC and 50oC, while xylanaseisolate 18 achieve its optimum production time at the 18th and 36th hours of incubation culture. Xylanase isolate 18 achieved its optimum condition at pH 5 and temperature 90oC and has half-life time 1 hour 7 minutes},
	keywords = {Jambi, Taman Nasional Bukit Duabelas, xilanase, Xilanolitik},
	author = {Kurrataa{\textquoteright}yun  Kurrataa{\textquoteright}yun}
}
