@conference {167,
	title = {Production of Methylripariochromene-A Bioactive Compound   at Eupatorium riparium Reg. by In Vitro Tissue Culture Technique },
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {Eupatorium riparium Reg.  have been reported that contain of methylripariochromene-A (MRC-A) and this compounds can be used to treat hypertension, anticancer, diuretic, decrease of sistolic/diastolic, and also were used in inhibiting colony of fungus and weeds. MRC-A can be produced by callus tissue culture with short and continuous production cycles. In general, high levels auxin (2,4-D) has been reported that  to be suitable for triggering callus induction in tissue culture.  This research aimed is to determine suitable combination of plant growth regulator (PGR) namely cytokinin (kinetin) and auxin (2,4D) and also to know  concentration level of MRC-A production at  E. riparium callus culture. The Method of this research was  tissue culture  technique with combination of cytokinin and auxin were 1ppm kinetin  and 2 ppm 2,4-D; 1 ppm kinetin and 3 ppm 2,4-D; 1 ppm kinetin and 4 ppm 2,4-D and combination of 1 ppm kinetin and 5 ppm 2,4-D,  and medium for callus induction using Murrashige-Skoog (MS). MRC-A concentration analysed using TLC-Densitometry. The result of study, based on statistic analysis showed that callus dried weight of E. riparium treatment with combination of cytokinin and auxin  were for 1 ppm kinetin and 2 ppm 2,4-D (0.092 g); 1 ppm kinetin and 4 ppm 2,4-D (0.069 g); 1 ppm kinetin and 5 ppm 2,4-D (0.039 g) and 1 ppm kinetin  and 3 ppm 2,4-D (0.037 g) and its significantly different between treatment. Methylripariochromene-A concentration of callus        E. riparium was higher than MRC-A from leaves of    E. riparium. The highest concentration of MRC-A base on analysed by TLC-Densitometry founded on callus 1 ppm kinetin and 2 ppm 2,4-D (0.98\%), others 1 ppm kinetin and 4 ppm 2,4-D (0.77\%); 1 ppm kinetin and 5 ppm 2,4-D (0.45\%); 1 ppm kinetin and 3 ppm 2,4-D (0.44\%) and leaf of E. riparium (0.42\%), respectively. The best combination of PGR in callus culture to produced MRC-A was 1 ppm kinetin and 2 ppm 2,4-D. This research suggested that MRC-A bioactive compound can be produced by tissue culture in vitro was added trigger using elicitors and precursors},
	keywords = {4-D), auxin (2, cytokinin (kinetin), Eupatorium riparium Reg., Methylripariochromene-A, TLC-Densitometry},
	author = {Linus Yhani Chrystomo and Aditya Krishar Karim and Issirep Sumardi and L. Hartanto Nugroho and Subagus Wahyuono}
}
@conference {246,
	title = {In Vitro Development Mikorrhiza Of  Gigaspora sp And Acaulospora sp With Carrot Plant},
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {Pot culture for mass production of mikorrhiza (AMF) is susceptible to contaminate with hyperparasitic fungy. Research purposes to determine (i) the germination of Gigaspora sp and sp Acaulospora sp in-vitro culture, (ii) determine the level of development of colonization mikorhiza and plant root carrots.  Material AMF  type of Acaulospora sp and Gigaspora sp sp. Media culture White (minimais nutrient media) or  MM and culture medium double (MSR), so and  carrot plants in vitro culture . Experimental design used RAL 2x2x6 factorial, with further test DMRT (P <0,05). Observed parameters, germination and development of Gigaspora sp and Acaulospora sp in vitro. The results that Gigaspora sp and Acaulospora sp can germinate in the in-vitro culture media MSR and MM. The development of the second colonization mikorrhiza in two types of media is quite good, with a form of external hyphae, internal hyphae, vesicles and sporulation. No significant differences both types of media interaction and Mikorrhiza. Conclusion that  Gigaspora sp and Acaulospora sp can be developed in vitro in MM and MSR media. For the second development mikorrhiza, MM media is relatively better than MSR media , and the interaction between media types and mikorrhiza did not differ significantly.},
	author = {Siti Chalimah and Mahajoeno Mahajoeno}
}
@conference {245,
	title = {Uji  Kualitasdan Kuantitas Selai Bunga Kembang Sepatu (Hibiscus rosasinensis) Dengan Penambahan Perasan Jeruk Siem(Citrus nobilisvar. Microcarpa)GULA DAN Maezena },
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {Selai merupakan makanan yang berbentuk semi padat dan biasa digunakan sebagai bahan tambahan makan roti ataupun sebagai isi roti kering. Tanaman kembang sepatu merupakan tanaman yang biasanya dikenal orang sebagai tanaman pagar saja ataupun tanaman hias.Tanaman kembang sepatu memiliki banyak manfaat dalam pengobatan, misalnya untuk mengobati bronchitis, demam, haid tidak teratur, dll, dan pada bagian bunga mengandung hibiscetin. Tujuanpenelitian untuk mengetahui kandungan vitamin C, gula dan estetika selai dengan uji organoleptik (warna, aroma, rasa, tekstur, dan daya terima). Desain percobaan digunakan Rancangan Acak Lengkap (RAL) 3 faktor, faktor pertama yaitu air perasan jeruk siem dengan dosis 15 ml (J1) dan 15 ml (J2), faktor kedua yaitu gula pasir 37,5 gram (G1) dan 50 gram (G2), dan faktor ketiga yaitu tepung maizena 2,5 gram (T1) dan 5 gram (T2). Parameter yang diukur kadar vitamin C, gula, dan organoleptik. Vitamin C tertinggi terdapat pada sampel perlakuan air perasan jeruk siem 15 ml, gula 50 gr, dan tepung maizena 2,5 gr (J2G2T1) dengan kadar vitamin C sebesar 5,16 mg/\%. Kadar gula terendah dan tertinggi pada perlakuan Air perasan jeruk siem (10 ml); gula (37,5 gr); tepung maizena (5 gr) (J1G1T2) dengan kadar 23,3mg/\% dan Air perasan jeruk siem (15 ml); gula (50 gr); tepung maizena (2,5 gr) (J2G2T1) , dengan kadar 74,4 mg/\%. Sedangkan hasil uji organoleptik respon yang sangat disukai masyarakat adalah sampel perlakuan J2G2T2 (air perasan jeruk siem 15 ml, gula pasir 50 gram, tepung maizena 5 gram) yang memiliki warna merah keunguan, aroma sedap, rasa asam kemanisan, tekstur yang agak lembut.Selai yang dihasilkan dari bunga sepatu sesuaidenganStandarNasional Indonesia (SNI).Simpulan Hasil penelitian menunjukkan bahwa kandungan vitamin C, kadar gula dan tepung maezena mempengaruhi kualitas dan kuantitas selai kembang sepatu.kadar vitamin C tertinggi dengan penambahan perasan jeruk siem 15 ml; gula 50 gr; tepung maizena 2,5 gr. Dari uji organoleptik selai paling disuka pada penambahan perasan jeruk siem 15 ml; gula 50 gr; tepung maizena 5 gr.},
	keywords = {JerukSiem, kualitas dan kuantitas selai, Selai, TanamanKembang Sepatu},
	author = {Siti Chalimah and Desyi Nur{\textquoteright}aini}
}
@conference {160,
	title = {Pemanfaatan Bakteri Asam Laktat Untuk Fermentasi Dan Preservasi Kakao},
	booktitle = {Seminar Nasional ke-22 Perhimpunan Biologi Indonesia},
	year = {2013},
	publisher = {Fakultas Biologi Unsoed},
	organization = {Fakultas Biologi Unsoed},
	address = {Purwokerto, Jawa Tengah, Indonesia},
	abstract = {Kakao merupakan  salah satu komoditas andalan sektor perkebunan. Tidak hanya berpotensi menambah pemasukan devisa negara tetapi juga menjadi sumber penghasilan utama bagi petani di banyak sentra kakao di Indonesia, namun mutu biji kakao yang dihasilkan belum seragam dan sesuai dengan SNI, sehingga menyebabkan rendahnya harga jual kakao dipasaran internasional. Salah satu peluang untuk meningkatkan mutu kakao adalah melalui pengembangan teknologi fermentasi dan preservasi biji kakao menggunakan biakan bakteri asam laktat (BAL) yang diisolasi dari produk fermentasi berbasis nira lontar. Beberapa biakan BAL yang berhasi diisolasi dari larutan fermentasi nira lontar teridentifikasi sebagai Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Lactobacillus plantarum dan Lactobacillus fermentum.  Hasil pengujian aktivitas antimikroba isolat BAL menunjukkan bahwa hanya L. fermentum dan L. plantarum yang efektif menghambat pertumbuhan mikroba kontaminan pada biji kakao. Isolat L. plantarum mampu menghasilkan asam laktat sebanyak 2,05\% dan hidrogen peroksida sebanyak 24,87g/ml, namun tidak menghasilkan bakteriosin. Isolat L. plantarum juga mampu mereduksi keberadaan mikroba patogen S. Typhimurium dan Aspergillus flavus sebesar 2 unit log pada konsentrasi 107-109 CFU/ml, sehingga memenuhi standar mutu kakao kering yang telah ditetapkan},
	author = {Ho Effie Cahyani and Joko Sulistyo and Sri Purwaningsih}
}
