TY - CONF T1 - Expression and Purification of Voltage Dependent Anion Channel 3 (VDAC3) recombinant protein in Eschericia coli T2 - Seminar Nasional ke-22 Perhimpunan Biologi Indonesia Y1 - 2013 A1 - Asmarinah A1 - Tri Panjiasih Susmiarsih A1 - Dwi Ari Pujianto A1 - Endang Winiati Bachtiar KW - male immunocontraception KW - Recombinant VDAC3 KW - sperm AB - Human Voltage Dependent Anion Channel 3 (VDAC3) gene in sperm was obtained by reverse transcription-PCR (RT-PCR) strategy and succesfull subcloned to pET 100/D-TOPO. Recombinant VDAC3 protein was expressed in Eschericia coli BL21 star and purified by Ni-NTA Agarose and its concentration was measured quantively. VDAC3 was suggested play a role in sperm motility and proposed as potential sperm antigen for male immunocontraception development JF - Seminar Nasional ke-22 Perhimpunan Biologi Indonesia PB - Fakultas Biologi Unsoed CY - Purwokerto, Jawa Tengah, Indonesia ER -